File:16. Биуретска рекција.ogg

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Summary

Description
English: Biuret reaction:

The presence of proteins in mushrooms can be proven by a Biuret reaction. A button mushroom or champignon (Agaricus bisporus) is used as an object in the current experiment.

A large fruiting body is cut in half. The first reagent, sodium hydroxide with a concentration of 1 mol/dm3 is applied using a clean pipette. A thin layer should cover the entire surface of the cut side of the mushroom. The second solution is applied with another pipette, copper (II) sulfate with concentration of 1 mol/dm3, again over the entire surface. Due to the presence of proteins, the color changes, from light-blue to dark-blue and purple. This change in color is due to the formation of a copper-protein complex.

Planned and performed by Slavica Tofilovska and Katerina Rusevska, Department of Biology, FNSM, Ss. Cyril and Methodius University, Skopje, Macedonia.
Македонски: Биуретска реакција:

Присуството на протеини во печурките може да се докаже со биуретска реакција, за што во овој експеримент се употребува шампињонот Agaricus bisporus.

Се зема едно големо плодно тело и се сече на половина. Со чиста пипета се зема од првиот реагенс, натриум хидроксид со концентрација 1mol/dm3 и внимателно се нанесува тенок слој на целата површина на пресечениот дел од печурката. Потоа со друга пипета се нанесува од вториот раствор, бакар (II) сулфат со концентрација 1mol/dm3, по целата површина. Поради присуството на протеини бојата се менува, од светло сина во темно сина и виолетова. Оваа промена на бојата се должи на формирањето на бакар-протеински комплекс.

Осмислено и изведено од Славица Тофиловска и Катерина Русевска, Институт за биологија, ПМФ, УКИМ, Скопје.
Date
Source Own work
Author Deni Ingilizovski

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29 September 2021

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